Everyday lab and bioprocess calcs: centrifuge speed and pelleting time, buffers at working temperature, dilutions and plate maps, primer melting, qPCR, cell culture planning, bioreactor oxygen transfer and scale-up, sterilisation and downstream steps. Each shows its method and checks itself against published cases.
Wired White v1 Screen LN₂ spill or boil-off oxygen depletion from inventory, room volume and ventilation. Compare a full-inventory spill with time-dependent room oxygen; local safety policy prevails.
Wired White v1 Plan exponential feeding, biomass and liquid volume for E. coli or yeast. Find when oxygen transfer, cooling, pump flow or vessel capacity requires a change.
Wired White v1 Size a Protein A column from titer, residence time and dynamic binding capacity. Estimate cycles, buffer use, process time and clean-bed pressure drop.
Wired White v1 Calculate TFF product yield, salt removal, buffer demand and membrane area. Includes gel-polarization flux, target diavolumes and the classical Cg/e diafiltration optimum.
Wired White v1 Estimate vial primary drying time, product temperature, and margin below collapse. Explore shelf temperature and chamber pressure with an animated ice front and a thermal design-space chart.
Wired White v1 Calculate RNA phosphate moles, N/P-based lipid stock volumes, and mixer flows. Track ethanol through mixing, dilution, and ideal equilibrium dialysis.
Wired White v1 Estimate F₀, spore log reduction, Del factor, SAL and nutrient retention through heat-up, hold and cooling. Explore how vessel volume changes the cycle.
Wired White v1 Calculate bioreactor scale-up at constant P/V, tip speed, mixing time or estimated kLa. Compare speed, power and trade-offs with animated tank views.
Wired White v1 Calculate oxygen solubility, kLa, OTR, oxygen demand and dry biomass limits. Compare oxygen capacity with jacket cooling duty and explore an animated dissolved-oxygen transient.
Wired White v1 Choose agarose percentage, estimate DNA band positions and run time, and mix a gel recipe. Animate the run or calibrate the approximate mobility model to your ladder.
Wired White v1 Estimate time to an induction OD600 from starting OD, doubling time and lag. Compare Gompertz and exponential growth, size the starter, and check measurement dilution.
Wired White v1 Calculate DNA primer melting temperatures with nearest-neighbor thermodynamics and Mg²⁺/dNTP corrections. Compare a primer pair, view melting curves, and plan a gradient PCR.
Wired White v1 Analyze Ct replicates with ΔΔCt and Pfaffl, geometric reference-gene normalization and propagated error. Fit standard-curve efficiency and calculate DNA copy numbers.
Wired White v1 Turn hemocytometer counts into viable cells/mL, fit population doubling time, and plan how many cells to seed for a chosen confluence day.
Wired White v1 Convert RPM and RCF at three sample radii, calculate rotor k-factor and estimate pelleting time. Transfer a protocol between rotors using their operating k-factors.
Wired White v1 Simulate strong, weak and diprotic acid–base titrations drop by drop. Find equivalence points, compare indicators and infer concentration from an endpoint.
Wired White v1 Calculate buffer masses or HCl/NaOH volumes for a target pH at working temperature. Includes Davies activity correction, buffer capacity and a titration curve.
Wired White v1 Plan serial dilutions with exact two-step routes, pipette limits, excess and dead volume. Map replicates on a 96-well plate and back-calculate CFU/mL.
A full-cycle engineering service provider & learning platform with focus on sustainable industry sectors with the aim to educate and support students and companies.
Copyright © 2026
Please confirm you want to block this member.
You will no longer be able to:
Please allow a few minutes for this process to complete.
