Alltägliche Rechner für Labor und Bioprozess: Zentrifugendrehzahl und Pelletierzeit, Puffer bei Arbeitstemperatur, Verdünnungen und Plattenbelegung, Primer-Schmelztemperatur, qPCR, Planung von Zellkulturen, Sauerstoffeintrag und Scale-up im Bioreaktor, Sterilisation und Aufarbeitung. Jeder zeigt seine Methode und prüft sich an veröffentlichten Fällen.
Wired White v4 Vorprüfung der Sauerstoffverarmung durch LN₂-Austritt oder Eigenverdampfung anhand von Vorrat, Raumvolumen und Lüftung. Vergleichen Sie den Austritt des Gesamtvorrats mit dem zeitabhängigen Sauerstoffgehalt im Raum; örtliche Sicherheitsvorgaben haben Vorrang.
Wired White v2 Plan exponential feeding, biomass and liquid volume for E. coli or yeast. Find when oxygen transfer, cooling, pump flow or vessel capacity requires a change.
Wired White v2 Size a Protein A column from titer, residence time and dynamic binding capacity. Estimate cycles, buffer use, process time and clean-bed pressure drop.
Wired White v2 Calculate TFF product yield, salt removal, buffer demand and membrane area. Includes gel-polarization flux, target diavolumes and the classical Cg/e diafiltration optimum.
Wired White v2 Estimate vial primary drying time, product temperature, and margin below collapse. Explore shelf temperature and chamber pressure with an animated ice front and a thermal design-space chart.
Wired White v2 Calculate RNA phosphate moles, N/P-based lipid stock volumes, and mixer flows. Track ethanol through mixing, dilution, and ideal equilibrium dialysis.
Wired White v2 Estimate F₀, spore log reduction, Del factor, SAL and nutrient retention through heat-up, hold and cooling. Explore how vessel volume changes the cycle.
Wired White v2 Calculate bioreactor scale-up at constant P/V, tip speed, mixing time or estimated kLa. Compare speed, power and trade-offs with animated tank views.
Wired White v2 Calculate oxygen solubility, kLa, OTR, oxygen demand and dry biomass limits. Compare oxygen capacity with jacket cooling duty and explore an animated dissolved-oxygen transient.
Wired White v2 Choose agarose percentage, estimate DNA band positions and run time, and mix a gel recipe. Animate the run or calibrate the approximate mobility model to your ladder.
Wired White v2 Estimate time to an induction OD600 from starting OD, doubling time and lag. Compare Gompertz and exponential growth, size the starter, and check measurement dilution.
Wired White v2 Calculate DNA primer melting temperatures with nearest-neighbor thermodynamics and Mg²⁺/dNTP corrections. Compare a primer pair, view melting curves, and plan a gradient PCR.
Wired White v2 Analyze Ct replicates with ΔΔCt and Pfaffl, geometric reference-gene normalization and propagated error. Fit standard-curve efficiency and calculate DNA copy numbers.
Wired White v2 Turn hemocytometer counts into viable cells/mL, fit population doubling time, and plan how many cells to seed for a chosen confluence day.
Wired White v2 Convert RPM and RCF at three sample radii, calculate rotor k-factor and estimate pelleting time. Transfer a protocol between rotors using their operating k-factors.
Wired White v2 Simulate strong, weak and diprotic acid–base titrations drop by drop. Find equivalence points, compare indicators and infer concentration from an endpoint.
Wired White v2 Calculate buffer masses or HCl/NaOH volumes for a target pH at working temperature. Includes Davies activity correction, buffer capacity and a titration curve.
Wired White v2 Plan serial dilutions with exact two-step routes, pipette limits, excess and dead volume. Map replicates on a 96-well plate and back-calculate CFU/mL.
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