Primer Tm and PCR Annealing Temperature Calculator
Wired White v1 Calculate DNA primer melting temperatures with nearest-neighbor thermodynamics and Mg²⁺/dNTP corrections. Compare a primer pair, view melting curves, and plan a gradient PCR.
Biotech and lab
Everyday lab and bioprocess calcs: centrifuge speed and pelleting time, buffers at working temperature, dilutions and plate maps, primer melting, qPCR, cell culture planning, bioreactor oxygen transfer and scale-up, sterilisation and downstream steps. Each shows its method and checks itself against published cases.
Wired White v1 
