Agarose Gel Simulator: Percent, Run Time, Band Positions
Choose agarose percentage, estimate DNA band positions and run time, and mix a gel recipe. Animate the run or calibrate the approximate mobility model to your ladder.
Calculators by WiredWhite engineers: inputs, formulas you can read, results, charts and drawings, checked against reference cases. They run in your browser.
Choose agarose percentage, estimate DNA band positions and run time, and mix a gel recipe. Animate the run or calibrate the approximate mobility model to your ladder.
Estimate time to an induction OD600 from starting OD, doubling time and lag. Compare Gompertz and exponential growth, size the starter, and check measurement dilution.
Calculate DNA primer melting temperatures with nearest-neighbor thermodynamics and Mg²⁺/dNTP corrections. Compare a primer pair, view melting curves, and plan a gradient PCR.
Analyze Ct replicates with ΔΔCt and Pfaffl, geometric reference-gene normalization and propagated error. Fit standard-curve efficiency and calculate DNA copy numbers.
Turn hemocytometer counts into viable cells/mL, fit population doubling time, and plan how many cells to seed for a chosen confluence day.
Convert RPM and RCF at three sample radii, calculate rotor k-factor and estimate pelleting time. Transfer a protocol between rotors using their operating k-factors.
Simulate strong, weak and diprotic acid–base titrations drop by drop. Find equivalence points, compare indicators and infer concentration from an endpoint.
Design low-pass and high-pass RC, RL, LC and Sallen-Key filters. Select E-series components and compare Bode plots, step response and cutoff tolerance bounds.
Compare stepper torque-speed estimates at maximum-torque alignment and driver voltages. Includes load inertia, L/R current rise, microstep torque and resonance.
Calculate RC wing area, loading, stall speed, MAC and CG. Compare tail volume and electric power targets, with a planform drawing and a separate flying-wing CG rule.
Calculate bolt circle hole coordinates and adjacent chord spacing from PCD, hole count and start orientation. Includes a numbered flange drawing, centre offsets and clockwise or counterclockwise numbering.
Calculate bend allowance, bend deduction and flat length for up to four bends. Compare formed and flat views, edit K-factor, and estimate air-bending force and minimum flange.
Check fillet weld throat stress, required leg size and weld group torsion under static loads. Includes elastic bending, AISC LRFD/ASD strength and size limits.
Calculate lathe or mill RPM, feed, metal removal, cutting power and torque. Compare machine limits using HSS/carbide starting speeds and chip-thickness-corrected cutting force.
Size a hydraulic power pack from pump displacement, pressure and cylinder geometry. Calculate push/pull force, speed, motor demand, reservoir volume, cooling duty and rod buckling.
Decode bearing numbers, bore sizes and suffixes. Calculate equivalent load, L10 life and the dynamic rating needed for a target life using your maker’s C rating.
Estimate wind pressure, face force and post-base overturning moment for signs, solid or porous fences and tilted solar mounts, with an editable embedded-post soil check.
Calculate irregular land area from side lengths and internal diagonals using Heron's formula. See a to-scale plot, check an extra diagonal, and convert to metric, US and local units.
Compare average end area and prismoidal earthwork volumes, include shrink and swell, and inspect a mass-haul diagram. A second mode estimates a building pad from a nine-point grid.
Calculate circular curve elements, PC/PT chainages and peg tables by exact Rankine deflections, tangent offsets and long-chord offsets. Choose 20 m, 30 m or 100 ft stationing.
Size a three-phase capacitor bank, compare star and delta capacitance, estimate tariff savings and screen resonance. Includes detuned reactors, power triangles and a motor no-load limit.
Compare solar DC/AC ratios, annual yield and added-panel payback. Separate MPPT current loss, inverter clipping and grid export curtailment, with an east–west option.
Size a pulse-induction coil, estimate resonance and damping, compare recovery with target decay, and explore calibrated air range versus coil diameter.
Size a two-switch forward welding inverter: transformer turns and copper, output choke, switch currents and losses, mains demand, and the arc load characteristic.
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